The basis of ELISA is the solid phase of antigen or antibody and the enzyme labeling of antigen or antibody. Antigens or antibodies bound to the surface of the solid carrier still maintain their immunological activity, and enzyme-labeled antigens or antibodies retain both their immunological activity and enzyme activity.
When testing, the tested substance (antigen or antibody) in the sample binds to the fixed antibody or antigen. Remove the non-conjugate by washing the plate, and then add the enzyme-labeled antigen or antibody. At this time, the amount of enzyme that can be fixed is related to the amount of the tested substance in the sample. By adding the substrate that reacts with the enzyme to develop color, the content of the substance in the sample can be judged according to the depth of the color, and qualitative or quantitative analysis can be carried out. Due to the high catalytic efficiency of the enzyme, the result of the immune response is indirectly amplified, and the determination method achieves high sensitivity.
Our Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, we use Molecular Device SpectraMax® iD5 reader to measures TRF and FP and also can expand to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.
Molecular Devices Microplate Reader
MSD is currently one of the most commonly used platforms in the field of macromolecular analysis. It combines a proprietary pattern array with electrochemical detection and has the following advantages:
- High sensitivity and wide linear range
- Save sample usage and achieve multiple detection.
- Homogeneity and high repeatability
- The sample is highly compatible and the matrix effect is small.
- The experimental process is simple, fast and diversified.
- The signal is stable and not affected by the color sequence
Due to the unique advantages of MSD, MSD platform is widely used in the detection and analysis of biomarkers, PK and immunogenicity. In the detection of biomarkers, MSD kits have various types and high sensitivity, which can be used for quantitative detection and analysis of various cytokines and chemokines, and multi-component detection of up to 10 components can be carried out. In PK detection, MSD can greatly expand the detection range of the standard curve, and ensure the accuracy and reliability of the detection results to the greatest extent with its high sensitivity and high stability. In the detection of immunogenicity, MSD platform has become the first choice for ADA and LBA NAb detection due to the high stability and discrimination of low-end signals.
MESO QUICKPLEX TM SQ120
Based on the principle of xMAP technology (liquid phase suspension chip technology), Luminex integrates multiple technologies such as fluorescent coded microspheres, laser detection, applied fluidics, the latest high-speed digital signals and computer algorithms, “High-throughput” testing is truly achieved, and it is the only high-throughput diagnostic technology that has been approved by the US FDA and is also the only high-throughput diagnostic technology that has been included in the US clinical laboratory quality control network, it has been evaluated as one of the trend technologies for clinical diagnosis by international industry experts. In the field of drug research and development, Luminex is widely used in the detection of cytokines, multiple pathogens, tumor markers, etc., and has the following advantages:
- High throughput: 1 detection, up to 100 indicators
- High speed: up to 10000 test/hour
- High sensitivity: the low detection limit can reach 0.01pg/ml
- Wide linear range: detection range up to 4-6 orders of magnitude
- Good repeatability: homogeneous reaction mode, each index has 1000-5000 reaction units, and the median mean value is taken for 100 times of analysis.