The QuantStudio®5 real-time fluorescence quantitative PCR system is a new product of the QuantStudio® system series. An advanced touch screen is used to make it easier for you to set up and run experiments. They are suitable for novice and experienced users. They are simple and cost-effective real-time fluorescence quantitative PCR systems, providing better performance and quality.
Obtain, analyze and share data anytime, anywhere-Use web browser-based software to remotely monitor your experimental operation, analyze complex data sets in a few minutes, and save the data in a safe space, share results safely online with colleagues across the child.
Obtain trusted results-Detect 1.5 times the copy number difference in a single analysis reaction to obtain a linear dynamic range of 10log.
In addition to flow cytometry, which can monitor the expression of CAR-T cells in subsequent vivo, it can also be detected by fluorescence quantitative PCR. TaqMan real-time fluorescence quantitative PCR is usually used to detect CAR-T cells in peripheral blood or bone marrow to determine the survival and distribution of CAR-T cells in patients after reinfusion. The real-time fluorescence quantitative PCR method has the characteristics of high specificity. In the field of cell therapy, the screening of virulent strains, the degree of virus droplets, the average vector copy number of the product, and the survival of the patient’s CAR-T cells after reinfusion, replicative retrovirus residue, host DNA residue and mycoplasma detection are widely used.
The method of qPCR was used to detect the cell therapy product and its clinical sample L for RCL (replication virus). In the 2018, the “Key Points for Quality Control and Testing of CAR-T Cell Therapy Products and Non-clinical Research Considerations” issued by the China Institute for Food and Drug Administration clearly stipulates the necessity of RCL testing.
Our company establishes RCL detection method based on qPCR platform, with quantitative sensitivity up to 2 copies/μl and qualitative sensitivity up to 1 copies/μl. The target gene and the internal reference gene can react at the same time, and RCL results can be quantified to the number of copies per ng DNA according to customer requirements.